Bacterial Detecting Dipslides
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Bacterial Detecting Dipslides
Total Bacteria Slides
Total Bacteria dipslide paddles, 10 per box. Dip slides have all the advantages of standard microbiology without any of the drawbacks.
- Simple to use.
- Easy to interpret.
- Reliable Two results in one test.
- Yeast & Bacteria testing.
- Results available in 24-48 hours.
- No lab facilities required
Bacteria grow as Red or colorless colonies. The Brown side will detect the presence of yeasts and/or fungi
Remove the cap from the bottle and withdraw the combined cap/tongue unit taking care not to touch the Culture Medium.
Press one medium against the surface to be tested.
Repeat the procedure with the other medium but do not use exactly the same spot. When testing curved surfaces roll the slide to ensure maximum contact between the Agar and the surface.
Remove the dip slide tongue from its bottle, holding it by the cap to avoid touching the culture medium.
Immerse it in the fluid to be tested for about ten seconds, or expose the slide to a spray or running fluid so that the agar surfaces are covered.
Remove the slide from the fluid and allow it to drain for a few seconds.
Replace the dip slide into its bottle and tighten the cap.
Inoculated dipslides should be incubated at 35°C. It is important to begin incubation as soon as possible, and to continue incubation for several days to guard against false negative results.
Incubation time is 1-2 days for bacteria. The optimum temperature for most ‘yeasts and moulds is 27°C to 30°C.
Incubation time 2-7 days.
If the incubation temperature is considerably different from the operating temperature, microbial growth may be slow and it is advisable to continue
incubation for further periods to detect the presence of organisms.
Interpretation of Results
Product – Description
- BT2 – Nutrient Agar with TTC. Most colonies show up as red spots. For general use in a wide variety of areas.
- PC2 – Plate Count agar; a good general growth medium suitable for all total count applications, but of particular use for dairy products
- PC2T – (As above) with TTC “Red Spot Dye”
- BTM2 – Nutrient Agar with TTC and Malt Extract Agar. Malt Extract Agar is a growth medium for yeasts and moulds which reduces bacterial growth. High levels of bacteria can overwhelm the suppression.
- RBS – Tryptone Soya Agar with TTC and Rose Bengal Agar. Rose Bengal is a selective agar for yeasts and moulds, however, the antibiotic content can cause interference with the total count reading.
- PO – A Plate Count Agar and OGYE Agar. OGYE is a selective agar using Oxytetracycline. This antibiotic is sensitive to temperature and should not be incubated at temperatures above 25 deg. C. Suitable for food microbiology, includes neutralisers for the most common germicides.
- BRB2 – Double Rose Bengal for yeasts and moulds only. Rose Bengal is taken up by small colonies, which show up as pink dots and are easier to count.
- SC2 – A plate count agar/ MacConkey No.3 Agar. MacConkey No.3 is a coliform specific medium that suppresses all non coliform bacteria. The coliform group includes most of the important causative organisms of food poisoning. The plate count agar gives a general count of viable aerobic organisms.
- SCN – Same as SC2, but including neutralisers for most common germicides. This slide improves the recovery of sub lethally damaged organisms, which could cause problems if left, but which do not normally show up on culture plates because traces of disinfectant have been transferred. This slide is most useful for assessing the effectiveness of cleaning procedures.
- SCT – Same as SC2, but with addition of TTC “Red Spot Dye” in the Plate Count medium
- PCV – Plate Count and VRBG Agar. VRBG is a bile based medium without lactose and is selective for Enterbacteriaceae. This group includes Coliforms. Plate Count is a general growth Agar
- TV – As PCV, but including neutralisers (see SCN/PO)
- TP – Nutrient Agar and Pseudomonas CFC Agar. For the detection of all pseudomonas species
- TT2 – Standard urine dipslide with CLED/MacConkey. Useful for a wide variety of screening test. Represents a considerable saving in time and money over standard MSU techniques. Dual Media slides give more information than single media types
- BV – Baird Parker Agar and VRBG Agar. For Staphylococci and Enterbacteriaceae
- E – VRBG Agar both sides
- BP – Baird Parker Agar both sides
|Dimensions||6 × 6 × 5 cm|
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